MEGAMER facility
Equipment List

Cytopeia Influx Three-Laser Flow Cytometer and high speed, bench-top cell sorter with three laser excitation paths, two scatter detectors and eight fluorescence detectors.

-Air-cooled Solid State "Sapphire" (Coherent), 200mW, 488nm laser, with 572/27, 531/40, 692/20 and 610/20 PMTs

-Air-cooled Solid State "Radius" (Coherent), 25mW, 635nm laser, with a 647 Longpass and a 700 dichroic diversion to 692/ and 715

-Air-cooled Solid State "Xcyte," 20mW, 355nm laser, covered with a 390 filter and a 610 dichroic division to 692/40 and 460/50 PMTs

Molecular Devices GenePix 4000B two laser, simultaneous scan microarray scanner with GenePix Pro 6.0 and Acuity 4.0 analysis software.

-17mW, 532 nm laser

-10mW, 635 nm laser

StepOne Real Time PCR 48 well unit, used both at sea and as a in-lab benchtop unit. Check iCal for availability.

Biorad MyCycler 96-well, gradient thermal cycler.

Fluid Imagine Technologies Flow Cam

-4 level magnification, scatter and fluorescence trigger

Beckman Coulter Z2 counter

Apple Xserve eight node, 16 processor, OSX based server equipped with the iNquiry bioinformatics software package developed and supported by the Bioteam, Inc.

-Nitrogen, Inquiry login page

Molecular Devices SpectraMax M2e Fluometric and Spectrophotometric plate and cuvette reader

-Dual momchromators- Absorbance 200 nm to 1000 nm; excitation between 250 nm and 850 nm.

Revco, Ultima II -80 C freezer

Biorad DCode Universal Mutation Detection System

Savant, model SC 210A modular multicomponent, VLP120 ValuePump driven Speed Vac system with a refrigerated vapor trap and 30 X 1.5 mL tube capacity.

Eppendorf, model 5804R benchtop, refrigerated centrifuge with 6 X 50mL, 6 X 15 mL and 30 X 1.5 mL rotor capacity.

Eppendorf, model 5410 benchtop micro centrifuge with 24 X 1.5 mL rotor capacity.

Olympus, model BH-2 three-color, epifluorescence microscope.

BioSpec variable-speed bead beater with an eight-tube capacity.

Mettler Toledo, model AG-204 Delta Range scale with a 0.1 mg detection limit.

Denver Instruments, model XS-410 scale with 0.10 g detection limit.

Owl, model B-1A 16-sample, model B2 40-sample and model D3 100-sample electrophoresis gel rigs.

UVP, model HB-500 microprocessor driven, variable speed Minidizer Hybridization Oven.

 

Maintained by: Brandon Carter

Last Updated: August 28, 2008